Chromatography and Other Separation Techniques

High Performance Liquid Chromatography/HPLC

 

Reversed phase chromatography

Normal phase chromatography

Chiral liquid chromatography

Size exclusion chromatography (GPC and gel filtration chromatography)

IC or IEC (ion exchange chromatography) using isocratic or gradient elution

 

Detection

VWD (variable wavelength detector)

DAD (diode array detector)

RI (refractive index detector)

Fluorescence detector

Light scattering detector

Electrochemical detector

Conductivity

MSD (mass sensitive detector)

 

Gas Chromatography/GC

Capillary gas chromatography (GC)

Headspace gas chromatography (static, dynamic) with FID, ECD and TCD

μGC

Chiral gas chromatography

 

Detection

FID (flame ionisation detector)

TCD (thermal conductivity detector)

ECD (electron capture detector) only for Head space GC

MSD (mass sensitive detector)

 

Thin Layer Chromatography (TLC)

Detection

Densitometry

 

Electrophoretic Techniques

Capillary electrophoresis (CE)
Capillary zone electrophoresis (CZE)
Chiral capillary electrophoresis
Micellar electrokinetic chromatography (MEKC)
Iisotachophoresis (ITP)
Isoelectric focusing (IEF)
Capillary gel electrophoresis (CGE)
Slab-gel electrophoresis (native gels and SDS-PAGE)

 

Detection

Ultraviolet absorbance diode array detection (UV DAD)
Laser induced fluorescence-detection (LIF)

Isoelectric focusing (PAGE; capillary electrophoresis)

 

Mass Spectrometry

Iontrap tandem mass spectrometry (MSn)
Matrix assisted laser desorption ionisation – time of flight - tandem mass Spectrometry (MALDI-ToF-ToF MS)
Inductively coupled plasma mass spectrometry (ICP-MS)